Modeling alveolar barrier disruption in vitro for sepsis-induced ARDS preclinical studies

European Respiratory Journal Nuria Roldan, Giulia Raggi, Kleanthis Fytianos, Jessica Petracca, Janick Stucki, Fabian Käsermann, Nina Hobi Abstract Disruption of the alveolar barrier is one of the main features of Acute Respiratory Distress Syndrome (ARDS), a devastating disease with no pharmacological treatments available at present. Sepsis is also a common risk factor for the development of ARDS, associated with 30% of the cases. During sepsis, hemolysis takes place,

More

Alveolar Dynamics and Beyond – The Importance of Surfactant Protein C and Cholesterol in Lung Homeostasis and Fibrosis

Frontiers in Physiology Kirsten Sehlmeyer, Jannik Ruwisch, Nuria Roldan, and Elena Lopez-Rodriguez Abstract Surfactant protein C (SP-C) is an important player in enhancing the interfacial adsorption of lung surfactant lipid films to the alveolar air-liquid interface. Doing so, surface tension drops down enough to stabilize alveoli and the lung, reducing the work of breathing. In

More

CD90+CD146+ identifies a pulmonary mesenchymal cell subtype with both immune modulatory and perivascular-like function in postnatal human lung

AJP Lung Cell and Molecular Physiology Limei Wang, Patrick Dorn, Soheila Zeinali, Laurène Froment, Sabina Berezowska, Gregor J. Kocher, Marco P. Alves, Melanie Brügger, Blandina I. O. Esteves, Fabian Blank, Carlos Wotzkow, Selina Steiner, Mario Amacker, Ren-Wang Peng, Thomas M. Marti, Olivier T. Guenat, Peter K. Bode, Ueli Moehrlen, Ralph A. Schmid, and Sean R.

More

Clinically relevant tissue scale responses as new readouts from organs-on-a-chip for precision medicine

Annual Review of Analytical Chemistry Olivier T. Guenat, Thomas Geiser & François Berthiaume  Abstract Organs-on-chips (OOC) are widely seen as being the next generation in vitro models able to accurately recreate the biochemical-physical cues of the cellular microenvironment found in vivo. In addition, they make it possible to examine tissue-scale functional properties of multicellular systems

More

Azithromycin has enhanced effects on lung fibroblasts from idiopathic pulmonary fibrosis (IPF) patients compared to controls

Respiratory Research  Kristina Krempaska, Sandra Barnowski, Jacopo Gavini, Nina Hobi, Simone Ebener, Cedric Simillion, Andrea Stokes, Ronja Schliep, Lars Knudsen, Thomas K. Geiser & Manuela Funke-Chambour  Abstract Background. Idiopathic pulmonary fibrosis (IPF) is a chronic fatal lung disease without a cure and new drug strategies are urgently needed. Differences in behavior between diseased and healthy

More

Air Space Distension Precedes Spontaneous Fibrotic Remodeling and Impaired Cholesterol Metabolism in the Absence of Surfactant Protein C

AJR Cell and Molecular Biology Jannik Ruwisch, Kirsten Sehlmeyer, Nuria Roldan, Begoña Garcia-Alvarez, Jesus Perez-Gil, Timothy E Weaver, Matthias Ochs, Lars Knudsen, Elena Lopez-Rodriguez Abstract Surfactant protein (SP)-C deficiency is found in samples from patients with idiopathic pulmonary fibrosis, especially in familial forms of this disease. We hypothesized that SP-C may contribute to fibrotic remodeling

More

Air space distension precedes spontaneous fibrotic remodeling and impaired cholesterol metabolism in the absence of Surfactant Protein C

American Journal of Respiratory Cell and Molecular Biology Jannik Ruwisch, Kirsten Sehlmeyer, Nuria Roldan, Begoña Garcia-Alvarez, Jesus Perez-Gil, Timothy E. Weaver, Matthias Ochs, Lars Knudsen & Elena Lopez-Rodriguez Abstract Surfactant protein (SP)-C deficiency is found in samples from patients with idiopathic pulmonary fibrosis, especially in familial forms of this disease. We hypothesized that SP-C may

More

Investigating the toxicological potential of inhaled aerosols in a breathing lung-on-chip

European Respiratory Journal Yara-Maria Proust, Nuria Roldan, Nicole Schneider-Daum, Claus Michael-Lehr, Hanno Huwer, Tobias Krebs, Olivier T. Guenat, Janick D. Stucki, Nina Hobi Abstract The widespread application of nanoparticles has drawn intense concerns about their impact on the environment and human health. The major route of nanoparticles into the human body are the alveolar ducts,

More

A lung-on-chip in vitro approach to study inflammation at the alveolar level

European Respiratory Journal Nuria Roldan, Aude Rapet, Giulia Raggi, Maxime Epars, Kleanthis Fytianos, Janick D. Stucki, Nicole Schneider-Daum, Claus Michael-Lehr, Hanno Huwer, Thomas Geiser, Olivier T. Guenat & Nina Hobi  Abstract Pulmonary inflammatory disease is a worldwide health burden. As a common hallmark, the integrity of the alveolocapillary barrier is compromised, causing infiltration of proinflammatory

More